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. Author manuscript; available in PMC: 2016 Aug 17.
Published in final edited form as: J Cell Physiol. 2015 Oct;230(10):2447–2460. doi: 10.1002/jcp.24975

Fig. 5.

Fig. 5

BCI-121 inhibits SMYD3 activity in vitro and in CRC cell models and affects cell proliferation. (A) In vitro methylation assay using the indicated recombinant SMYD3 protein on a mixture of calf thymus histones in the presence and/or absence of BCI-121 showing a significant decrease in H4 methylation. Autoradiograph and Coomassie stained (loading control) gels are shown. BCI-121 inhibits cell proliferation in HT29 (B) and HCT116 (C) cells in a dose- and time-dependent manner. Cell proliferation was assessed by cell counting. The data presented are the mean values obtained for each analyzed time point (n = 4). (D, E) BCI-121 100 μM decreases the expression levels of SMYD3 target genes in both cell lines (the 48 h time point was evaluated). β-actin was used for normalization of real-time PCR data. Statistical analysis was performed using Student’s t-tail test; *P < 0.05, **P < 0.01, and ***P < 0.001 were considered statistically significant.