Fig. 7.

SMYD3 is required for proper ovarian cancer cell growth. (A) SMYD3 genetic ablation in ovarian cancer cell models decreases the global level of targeted histone methyl marks [H3K4me2 and H4K5me] without affecting a non-targeted methyl mark [H3K27me3]. (B) SMYD3 genetic ablation levels correlate with deregulated mRNA expression of its target genes and (C) with decreased cell proliferation rate. OVCAR-3 and SKOV-3 cells were transfected with control or SMYD3-specific siRNAs for 48 h and 96 h. Levels of methyl marks were determined by immunoblot; expression of target genes was analyzed by real-time PCR (the 48 h time point was evaluated). Cell proliferation was assessed by cell counting. The number of cells for each indicated time point is plotted. β-actin was used as a loading control for immunoblotting and for normalization of real-time PCR data. Statistical analysis was performed using Student’s t-tail test; *P < 0.05, **P < 0.01, and ***P < 0.001 were considered statistically significant.