Table 1.
Antibodies and stains used for immunohistochemistry
| Primary antibodies | |||||
|---|---|---|---|---|---|
| Specificity | Cells labelled | Source (isotype) | Clone | Company | Working concentration |
| NeuN | Neurons | Mouse (IgG2a) | Monoclonal | Abcam | 5 μg/mL |
| TUJ1 | Neurons | Mouse (IgG2a) | Monoclonal | Covance | 5 μg/mL |
| Thy1.2 | RGCs | Rat (IgG2b) | Monoclonal | Abcam | 2.5 μg/mL |
| Active caspase‐3 | Apoptotic cells | Rabbit | Polyclonal | Chemicon (Millipore) | 10 μg/mL |
| Secondary antibodies and nuclear stain | ||||
|---|---|---|---|---|
| Specificity | Conjugate | Source | Company | Working concentration |
| Mouse IgG | AlexaFluor 488 | Goat | Invitrogen – Molecular Probes Inc. | 4 μg/mL |
| Rat IgG | AlexaFluor 488 | Goat | Abcam | 4 μg/mL |
| Rabbit IgG | AlexaFluor 488 | Goat | Invitrogen – Molecular Probes Inc. | 4 μg/mL |
| TO‐PRO‐3 iodide 642 | Invitrogen – Molecular Probes Inc. | 1 μm | ||