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. Author manuscript; available in PMC: 2016 Aug 17.
Published in final edited form as: Biochemistry. 2016 Jan 14;55(3):447–458. doi: 10.1021/acs.biochem.5b00936

Figure 2.

Figure 2

Biochemical changes of BaL.01 pseudo-viruses caused by MβCD treatment: (A) change in HIV-1 envelope cholesterol content normalized to untreated envelope following MβCD treatment (cholesterol was measured fluorometrically with Amplex Red (n = 2, mean ± SD)), and (B) quantification of HIV-1 spike proteins that are retained after MβCD treatment (gp120 (black squares) was assayed from the supernatant and retained gp41 (red circles) was assayed from the virus pellet fraction (gp120: n = 2, gp41: n = 4, mean ± SD)).