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Upper row: fluorescence responses of fluorescent probes A, B and C (5 μM) to pH at 4.0 and 7.4 in the absence and presence of various metal ions (200 μM) in buffer solutions (with 1% DMSO as co-solvent). Lower row: fluorescence intensities changes of fluorescent probes A, B and C (5 μM) as a function of time in 2 hours under excitation in buffer solutions (1% DMSO as co-solvent) at pH 4.0 and 7.4.
