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. Author manuscript; available in PMC: 2016 Aug 17.
Published in final edited form as: Thromb Haemost. 2014 Jul 24;112(4):770–780. doi: 10.1160/TH14-01-0043

Figure 6. Analysis of the RNA integrity and RT-PCR.

Figure 6

A) An electrophoretic trace of a representative sample of tRNA isolated from 30,000 circulating endothelial cells (CECs) collected by fluorescence-activated cell sorting. B) An electrophoretic trace of total RNA isolated from 20,000 human umbilical vein endothelial cells (HUVECs) recovered by fluorescence-activated cell sorting. C) Only the HUVECs were positive for all four endothelial cell mRNAs. A small amount of Von Willebrand factor (vWF) message was found in two of the CEC RNA preparations tested. For each sample, 10 µl from a 25 µl PCR reaction was evaluated.