Table 1.

Plasmids and other available vectors for CRISPR/Cas9 delivery. 2A, 2A viral ribosomal skipping sequence; Puro, puromycin resistance gene; SpCas9, Streptococcus pyogenes Cas9; SpCas9n, Streptococcus pyogenes Cas9 D10A nickase.

Plasmid	Addgene ID	Cas9 Version	Additional Elements	Reference
pX330	42230	SpCas9	N/A	(Cong et al., 2013)
pX335	42335	SpCas9n	N/A	(Cong et al., 2013)
pX458	48138	SpCas9	2A-EGFP	(Ran et al., 2013b)
pX459	62988	SpCas9	2A-Puro	(Ran et al., 2013b)
pX460	48873	SpCas9n	N/A	(Ran et al., 2013b)
pX461	48140	SpCas9n	2A-EGFP	(Ran et al., 2013b)
pX462	62987	SpCas9n	2A-Puro	(Ran et al., 2013b)
eSpCas9(1.1)	71814	eSpCas9	N/A	(Slaymaker et al., 2016)
lentiCRISPR v2	52961	SpCas9	Lentiviral elements	(Sanjana et al., 2014)