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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.6.1–5B.6.60. doi: 10.1002/cpsc.15
1. 95°C 10 minutes
2. 95-85°C −2°C per second
3. 85°C 1 minute
4. 85-75°C −0.3°C per second
5. 75°C 1 minute
6. 75-65°C −0.3°C per second
7. 65°C 1 minute
8. 65-55°C −0.3°C per second
9. 55°C 1 minute
10. 55-45°C −0.3°C per second
11. 45°C 1 minute
12. 45-35°C −0.3°C per second
13. 35°C 1 minute
14. 35-25°C −0.3°C per second
15. 25°C 1 minute
16. 25-4°C −0.3°C per second
17. 4°C hold