| 1. 98°C | 30 seconds |
| 2. 98°C | 10 seconds |
| 3. 66°C | 20 seconds |
| 4. 72°C | 20 seconds |
| 5. GOTO step 2, 20 times | |
| 6. 72°C | 3 minutes |
| 7. 4°C forever |
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