| 5.4 μL | ddH2O |
| 2.0 μL | GC Buffer (5x) |
| 0.3 μL | DMSO |
| 0.2 μL | dNTPs (10 mM) |
| 0.5 μL | Forward Primer (10 μM) |
| 0.5 μL | Reverse Primer (10 μM) |
| 1.0 μL | Purified genomic DNA (protocol 1.8.1) from the hPSC line to be targeted (100 ng/μL) |
| 0.1 μL | Phusion Hot Start II DNA Polymerase |
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