| 54 μL | ddH2O |
| 20 μL | GC Buffer (5x) |
| 3 μL | DMSO |
| 2 μL | dNTPs (10 mM) |
| 1 μL | Phusion Hot Start II DNA Polymerase |
| 5 μL | Forward Primer (10 μM) |
| 5 μL | Reverse Primer (10 μM) |
| 10 μL | Plasmid DNA diluted to 1 ng/uL |
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