4.4 μL	ddH2O
2.0 μL	GC Buffer (5x)
0.3 μL	DMSO
0.2 μL	dNTPs (10 mM)
0.1 μL	Phusion Hot Start II DNA Polymerase
0.5 μL	Forward Primer (10 μM)
0.5 μL	Reverse Primer (10 μM)
2.0 μL	DNA (pick a single colony into 50 μL LB)