| 1. 12.5 μL | ddPCR supermix |
| 2. 0.225 μL | Forward primer (100 μM) |
| 3. 0.225 μL | Reverse primer (100 μM) |
| 4. 0.08 μL | Reference probe (100 μM) |
| 5. 0.08 μL | Alternate (edit-specific) probe (100 μM) |
| 6. x μL | Sample (this is typically 1 μl for a confluent well of a 96-well plate digested with 50–100 μl HotShot or DirectPCR/ProK buffer) |
| 7. Bring to 25 μL | ddH2O |
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