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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.6.1–5B.6.60. doi: 10.1002/cpsc.15
15 μL Gibson Assembly Mix
100 ng Vector Backbone
100 ng Reporter/Selection Cassette
100 ng 5′ Homology Arm
100 ng 3′ Homology Arm
x μL ddH2O

20 μL Total