1 μL | Oligo 1 (100 μM) |
1 μL | Oligo 2 (100 μM) |
1 μL | 10x T4 DNA Ligase Buffer |
6.5 μL | ddH2O |
0.5 μL | T4 PNK |
| |
10 μL | total |
1 μL | Oligo 1 (100 μM) |
1 μL | Oligo 2 (100 μM) |
1 μL | 10x T4 DNA Ligase Buffer |
6.5 μL | ddH2O |
0.5 μL | T4 PNK |
| |
10 μL | total |