| 1 μL | 50 ng BbsI-digested and dephosphorylated expression plasmid from step 1 (diluted to 50 ng/μL) |
| 1 μL | phosphorylated and annealed oligo duplex from step 2 (1:200 dilution) |
| 5 μL | 2x Quick Ligation Buffer |
| 2 μL | ddH2O |
| 1 μL | Quick Ligase |
|
| |
| 11 μL | total |
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