| T7 10x Reaction Buffer | 1 μL |
| ATP Solution | 1 μL |
| GTP Solution | 1 μL |
| CTP Solution | 1 μL |
| UTP Solution | 1 μL |
| ddH2O | 2 μL |
| Oligo template | 2 μL |
| T7 Enzyme Mix | 1 μL |
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