| ddH2O | 31 μL |
| GC Buffer | 10 μL |
| dNTPs | 1.0 μL |
| For Primer | 2.5 μL (10 μM) |
| Rev Primer | 2.5 μL (10 μM) |
| Human genomic DNA | 1.0 μL (250 ng/μL) |
| DMSO | 1.5 μL |
| Phusion Hot Start II* | 0.5 μL |
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