Fig. 4.

White matter atrophy and Lewy body pathology. (A) Luxol fast blue staining of a whole brain slice from the rostral part of the brain showing white matter atrophy (asterisks) and enlarged lateral ventricles (LV). (B and C) Phosphorylated (S129) α-synuclein staining showing the presence of Lewy bodies in the grafted cells (C) and in the host putamen surrounding the graft (B). (D–M) Quantification of phosphorylated α-synuclein showing that up to 12% of neuromelanin-containing cells contain Lewy bodies. Extensive Lewy pathology appears in different brain regions, including amygdala (D and I), cortex (E and J), hippocampus (F and K), substantia nigra (G and L) and the nongrafted putamen (H and M). (N–P) Confocal images illustrating a host nigral dopaminergic neuron (red, TH positive) containing a typical Lewy body (green, α-synuclein positive). (Q) Ubiquitin-positive inclusions also appeared in the grafted cells. Up to 11% of the neuromelanin-containing cells contained ubiquitin-positive aggregates. (Scale bars: 10 mm in A, 20 µm in B and C, 50 µm in D–H, 20 µm in I–Q.)