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. 2016 May 23;113(23):6490–6495. doi: 10.1073/pnas.1523157113

Fig. S2.

Fig. S2.

pT173 antibody recognizes RUNX1, RUNX2, and RUNX3 during mitosis. (A) Immunoblot comparing RUNX1, RUNX2, and RUNX3 expression in COLO201, DLD1, and SNU5 cells. A total of 30 µg of total cell lysate was loaded per lane. (B) Immunofluorescence microscopy with pT173 antibody. Cells were imaged by confocal microscopy (Olympus FluoView FV1000). Red, pT173; green, γ-tubulin; blue, DNA. (C) Asynchronous DLD1 cells were stained as in B. Arrows indicate cells at different stages of mitosis. (Scale bars, 10 µm.)