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. 2016 May 23;113(23):E3290–E3299. doi: 10.1073/pnas.1606348113

Fig. S4.

Fig. S4.

PI(4)P recovery was slowed by SMIT1 and myo-inositol. Related to Fig. 4. (A) Cartoon showing the P4M-YFP and Lyn11-FRB-CFP as a FRET pair used to monitor the PI(4)P metabolism at the plasma membrane. P4M-YFP specifically binds to PI(4)P. (B) Representative whole-cell photometric FRET ratio changes using the fluorescent tools described in A, after applying Oxo-M with and without myo-inositol and SMIT1. (C) Summary of the data in B, highlighting the effects of myo-inositol and SMIT1 on the percentage of recovery 300 s after the FRET ratio was fully reduced (n = 4–6). Data are means ± SEM.