Fig. S1.

Depletion of OGT does not affect deposition of histone H3.1. (A) Knockdown efficiency of five shRNA targeting OGT. Lentivirus for each of the five shRNAs targeting OGT, as well as a nontargeting control were transduced into HEK293T cells. Knockdown efficiency was monitored by Western blot. (B–E) OGT depletion has no apparent effect on de novo deposition of histone H3.1. HeLa cells expressing H3.1-SNAP cells were transduced with shRNA targeting OGT or CAF-1p150 (control). Nucleosome assembly of new H3.1 was monitored by using SNAP staining of individual cells (B and C) or chromatin fractionation (D and E) 72 h after selection. (Scale bar: 20 µm.) Values represent mean ± SEM (*P < 0.05; **P < 0.01; n = 3 biological replicates; Student’s t test).