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. 2016 May 23;113(23):E3231–E3239. doi: 10.1073/pnas.1606381113

Fig. S4.

Fig. S4.

Effect of the acceptor position in the geometric model for BK-LBT constructs in the presence of the β1-subunit. (A) The z-coordinate of all donors except S1 clash with the upper boundary equal to the height of the acceptor (37.4 Å) when the acceptor radial shift is equal to 0, which corresponds to the best acceptor position for the data in absence of the β1-subunit (4.75 Å radial from the pore axis). We thus explored the possibility of a slight β1-induced acceptor perturbation by varying the acceptor radial distance to achieve a more structurally meaningful spread of z-coordinates among LBT constructs. A positive shift means further away from the z axis. When acceptor radial shift is >1 Å, the S1 donor drops below the extracellular face of the membrane (15 Å, dashed line). (B) Fit error (χ2RG) vs. acceptor radial shift. χ2RG decreases for all constructs as the acceptor is displaced away from the z axis. The average fit error of the entire dataset is shown as black diamonds.