Fig. 3.
Cellular transfer of FACS-purified allogeneic MCs shows that the resorption response can be adoptively acquired. (A) Allogeneic MCs were prospectively isolated by FACS (populations 4 and 5), transferred via ampullar injection into recipients, and followed for features of allogeneic resorption. (Top) Time lapse imaging over 24-h intervals of a recipient colony after receiving 1 × 105 allogeneic MCs (red arrow indicates site of cellular transfer, followed by parental zooid and bud elimination). (B and C) Kaplan–Meir survival curves among recipient colonies show that transfer of allogeneic MCs eliminates recipient primary buds and zooids compared with non-MC and mock injected groups (P < 0.0001 by Log-rank test). (D) Transfer of 1 × 105 allogeneic control MC-less cells (small lymphocyte-like cell; population 1) had only minimal effects on recipient colonies. (E and F) Timing of bud and parental zooid loss in representative MC (E) and non-MC (F) recipients. (G) Box and whisker plots among groups showing the ratio of buds to parental zooids over time. Transfer of allogeneic MCs eliminates recipient primary buds, resulting in a decline in the bud-to-zooid ratio from 1.7 (±0.03) to 0.9 (±0.06) (P = 0.0001).