Fig. 4.

RNA-seq analysis of allogeneic resorbing colonies. (A) Schematic of RNA-seq experiments. Allogeneic, but compatible, defined lines were fused in colony allorecognition assays and then harvested for RNA-sequencing. Biologic replicates were obtained from stage R = II (resorption score = II) 1° (primary) and 2° (secondary) buds, corresponding to developmental stage D. (B) A hierarchical clustering matrix was generated using Pearson correlation coefficients from log₂ counts per million across all genes. Widely divergent transcriptomes were observed across resorption status and developmental stage. The color scale indicates the degree of correlation (white, low correlation; purple, high correlation). (C) Pie chart showing blastogenic stage at time of chimeric resorption.