Fig. S7.

A-type K⁺ channels regulated slow excitatory potentials induced by infusing d-myo-inositol trisphosphate into a nearby astrocyte. (A) Schematic depicting experimental design associated with data presented in this figure. Two sets of experiments (same as Fig. 6), one with and the other without 20 µM 3,4–DAP (A-type K⁺ channel blocker) in the recording pipette, were performed. Color codes for each dataset are shown and are the same for B–K. (B) Representative trace of a high-amplitude long-duration SEP when A-type K⁺ channels were blocked. This trace was recorded after infusion of InsP₃ into a nearby astrocyte. (C) Normalized cumulative histogram of SEP amplitude before (PRE) and after (POST) infusion of InsP₃ into a nearby astrocyte, after A-type K⁺ channels were blocked with 3,4–DAP. (D and E) SEP peak amplitude (D; mean ± SEM) and normalized cumulative histogram of amplitudes of all recorded SEPs (E) plotted for cases where A-type K⁺ channels were present (Control) or blocked (3,4–DAP), after infusion of InsP₃ into a nearby astrocyte. (F) Normalized cumulative histogram of SEP 20–80% rise time, with the experimental configuration described in C. (G) Normalized cumulative histogram of SEP 20–80% rise time, with the experimental configuration described in E. (H) Normalized cumulative histogram of SEP FWHM duration, with the experimental configuration the same as in C. (I) Normalized cumulative histogram of FWHM, with the experimental configuration the same as in E. (J and K) SEP frequency (J) and peak SEP frequency (K) before (PRE) and after (POST) infusion of InsP₃ into a nearby astrocyte, after A-type K⁺ channels were blocked with 3,4–DAP. For J and K solid rectangles represent the mean values. The data represented in E, G, and I are the same as in Fig. 6 C, D, and E, respectively. For K, P values correspond to a Mann–Whitney test.