Fig. 5.
Epoxygenase inhibition alters the resolution phenotype of recruited cells of the monocyte lineage. Inflammation was initiated by zymosan (1 mg, i.p.), and mice were treated with vehicle control (PBS) or epoxI (30 mg/kg, i.p.) at 24 and 36 h. Cells were collected and pooled from n = 10 mice and Ly6chi, Ly6clo monocytes, resident macrophages, and recruited dendritic cells were sorted on a FACSAria as detailed in Materials and Methods. A qRT-PCR resolution monocyte panel (n = 3–6) was then used to examine the phenotype of each cell type. (A–C) In the presence of epoxI, (A) Plxcd2, Ccna2, Ccnb2, Aspa, F5, Tgfb2, and Timd4 were found to be down-regulated in the cell types; (B) Ccr2, CCL2, Ms4A7, CD86, and IL1F9 were up-regulated in the cell types; and (C) Stfa2l1 was up- and down-regulated in a cell type-specific manner. *P < 0.05 by unpaired t test. (D, Upper) Summary of the relative basal levels of each transcript in each cell type. (D, Lower) Summary of the effect of epoxI on the different transcripts. Data represents mean ± SEM.