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. 2016 May 25;113(23):E3240–E3249. doi: 10.1073/pnas.1521453113

Fig. 6.

Fig. 6.

Epoxygenase inhibition regulates monocyte and macrophage differentiation. Inflammation was initiated by zymosan (1 mg, i.p.), and mice were treated with vehicle control (PBS) or epoxI (30 mg/kg, i.p.) at 24 and 36 h. Cells were collected at 48 h and pooled from n = 9–18 mice. Ly6c+ monocytes, resident macrophages, and monocyte-derived macrophages were sorted on a LSR Fortessa as detailed in Materials and Methods. (A) Representative zebra plots of the cell populations from control and epoxI-treated mice expressing Ly6c and F4/80, with labeled populations representing A: Ly6c+ monocytes; B: resident macrophages (Resi-Mϕ); and C: monocyte-derived macrophages (Mono-Mϕ). (B) Representative dot plots of the Ly6c+ monocytes, resident macrophages, and monocyte-derived macrophages from control and epoxI-treated mice expressing PKH26 and CD11b (Upper) and CD64 and MerTK (Lower). (C) Changes in cell numbers, and expression (MFI in relative fluorescence units; RFUs) for F4/80, CD11b, MHCII, CD103, CD64, MerTK, and Timd4 in the Ly6c+, Resi-Mϕ, and Mono-Mϕ cell populations. Data are mean ± SEM from n = 5 mice per group; *P < 0.05 by unpaired t test.