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. 2016 May 25;113(23):E3240–E3249. doi: 10.1073/pnas.1521453113

Fig. 7.

Fig. 7.

Epoxygenase inhibition reduces the phagocytic activity of monocyte-derived and resident macrophages. Inflammation was initiated by zymosan (1 mg, i.p.), and mice were treated with vehicle control (PBS) or epoxI (30 mg/kg, i.p.) at 24 and 36 h. Cells were collected at 48 h and pooled from n = 9–18 mice. Ly6c+ monocytes, resident macrophages, and monocyte-derived macrophages were sorted on a FACSAria as detailed in Materials and Methods. Sorted cells were then tested for their ability to phagocytose CFSE-labeled apoptotic cells (thymocytes) or FITC-labeled zymosan BioParticles over 30 min. Cells were gated using F4/80 or Ly6c on an ImageStreamX Mark II. (A) ImageStream analysis differentiates cells that phagocytose apoptotic cells or zymosan (internalization score >0) from those where particles or bodies just stick to the cell (internalization score 0). (B) Ex vivo phagocytosis of apoptotic cells (Left) and zymosan (Right) by Ly6c+, Resi-Mϕ, and Mono-Mϕ cell populations from control and epoxI-treated mice. Data are mean ± SEM from n = 3–6 pooled samples; *P < 0.05 by unpaired t test.