Table 3.
Primer sequences of chicken β-actin, copper-zinc superoxide dismutase (CuZnSOD), metallothioneins (MT), fatty acid synthetase (FAS), malate dehydrogenase (MDH) and lipoprotein lipase (LPL).
| Gene | Genbank Accession | Primer (5’-3’) | Product (bp) | Tm (°C)1 |
|---|---|---|---|---|
| β-actin | NM_205518.1 | F: GAGAAATTGTGCGTGACATCA | 152 | 60 |
| R: CCTGAACCT CTCATTGCCA | ||||
| CuZnSOD | NM_205064 | F: GGAGGAGTGGCAGAAGT | 159 | 55 |
| R: TAAACGAGGTCCAGCAT | ||||
| MT | NM_205275.1 | F: GCAACAACTGTGCCAAGGGC | 138 | 61 |
| R: TTTCGTGGTCCCTGTCACCC | ||||
| FAS | J03860 | F: AGCGTGCTATGCTTGCC | 129 | 60 |
| R: GTCCGTGACGAATTGCTTTAT | ||||
| MDH | XM_419350 | F: AAAGACTGTGAAGGTGGTAG | 114 | 56 |
| R: ATCCAAGCGAGTCAAGC | ||||
| LPL | NM_205282 | F: TACAGTCTGGGTGCTCAT | 104 | 56 |
| R: GCATACTCAAAGGTGGG |
1Annealing temperature.