. 2015 Oct 23;94(11):2686–2694. doi: 10.3382/ps/pev251

Table 8.

Effects of dietary Zn source and level on gene expressions of some fat metabolism-related enzymes in liver and abdominal fat of broilers at 42 days of age.

		Liver		Abdominal fat
	Added Zn
Zn source	level (mg/kg)	FAS mRNA (RQ)⁵	LPL mRNA (RQ)⁵	MDH mRNA (RQ)⁵	LPL mRNA (RQ)⁵
Control¹	0	0.840	1.12	0.967	1.04
ZnSO₄.7H₂O¹	60	1.242	1.80	1.310	1.29
	120	1.358	1.61	1.328	1.38
	180	1.410	1.33	1.385	1.29
Zn-AA W¹	60	1.093	1.58	1.090	1.44
	120	1.148	1.62	1.210	1.27
	180	1.258	1.28	1.153	1.38
Zn-Pro M¹	60	1.235	1.76	1.252	1.40
	120	1.437	1.93	1.223	1.31
	180	1.353	1.73	1.190	1.11
Zn-Pro S¹	60	1.278	1.47	1.253	1.15
	120	1.505	1.86	1.333	1.34
	180	1.340	1.49	1.085	1.34
Pooled SE		0.168	0.193	0.119	0.121
Zn source²	ZnSO₄.7H₂O	1.21	1.46	1.25	1.25
	Zn-AA W	1.09	1.40	1.11	1.28
	Zn-Pro M	1.22	1.64	1.16	1.22
	Zn-Pro S	1.24	1.49	1.16	1.22
Pooled SE		0.0843	0.0965	0.0595	0.0607
Zn level	0¹	0.840^a	1.12^a	0.967^a	1.04^a
	60³	1.212^b	1.65^b	1.226^b	1.32^b
	120³	1.362^b	1.76^b	1.274^b	1.32^b
	180³	1.340^b	1.46^b	1.203^b	1.28^b
Pooled SE		0.0843	0.0965	0.0595	0.0607
		P-value⁴
Source		0.559	0.370	0.404	0.839
Level		0.001	0.001	0.002	0.003
Source × level		0.996	0.886	0.943	0.745

^a,bMeans with different superscripts within the same column differ significantly (P < 0.05).

¹Each value represents the mean of 6 replicate cages with 6 chicks per cage (n = 6).

²Each value represents the mean of 18 replicate cages with 6 chicks per cage (n = 18).

³Each value represents the mean of 24 replicate cages with 6 chicks per cage (n = 24).

⁴Probability values for main effects of ANOVA.

⁵The mRNA abundances of enzymes were calculated as the relative quantities (RQ) of enzyme mRNA to β-actin mRNA; RQ = 2^−ΔΔCt (Ct = threshold cycle).