Figure 7.

Proportions of BrDU labelled cells from three strains of chicken MSCs (A) Broiler Ross 308, (B) SPF layer, Lohmann LSL and (C) Hy-Line layer cultured in the presence or absence of 1,25(OH)₂D₃ and osteogenic inducing factors. Cells were pulsed with BrDU (10mg/ml) for 2 h and the effect of 1,25(OH)₂D₃ on cell proliferation examined by quantitating proportions of BrDU labelled cells on Day 3 or 5 of osteogenic differentiation. Numbers of BrDU-positive cells were reduced in all strains examined, in the presence of both 2.4nM and 24nM concentrations of 1,25(OH)₂D₃ on Day 3 and Day 5 of osteogenic induction compared cells cultures in induction media alone (IM). A relative decrease in BrDU positive cells was also observed in cell cultures in the absence of osteogenic induction factors. Values are expressed as mean ± SEM. Broiler (n+4), SPF layer (n = 3), Hy-Line layer (n = 2). Comparisons marked with an asterisk (*) are significantly different (P < 0.05).Abbreviations: CM, control media containing no exogenous 1,25(OH)₂D₃; IM, induction media; IM+2.4nM, induction media containing 2.4nM 1,25(OH)₂D₃; IM+24nM, induction media containing 24nM 1,25(OH)₂D₃.