Fig 3. Whole cigarette smoke exposure induces phosphorylation of both EGFR and Erk1/2 that is inhibited by Gefitinib.

Differentiated NHBE cells were treated with air or WCS from 2 cigarettes with and without Gefitinib. After 24 h total cell protein lysates were prepared and equal amounts of protein were separated by SDS-PAGE, transferred to PVDF membranes and probed with anti-phospho-EGFR (Tyr1068) (A) or anti-Phospho-Erk1/2 (Thr202/Tyr204) (C) antibodies and anti-β actin antibody as a loading control (A, C). Graphs of the quantification of the signals for phospho-EGFR (B) from 3 different lungs and for phospho-Erk1/2 from 5 different lungs (D) are shown. * P<0.05, one-way ANOVA.