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. 2016 Aug 17;11(8):e0160216. doi: 10.1371/journal.pone.0160216

Fig 5. Inhibition of EGFR signaling restores ciliated cell differentiation during whole cigarette smoke exposure.

Fig 5

(A–F) Representative confocal micrographs of human airway epithelial cells after treatment with Air (A, D), Smoke (B, E) or Smoke + Geftinib (C, F) during 21 d of differentiation using ALI conditions, stained for FoxJ1 (green), acetylated-tubulin (white) or nuclei (blue) (A, B, C). Panels D, E and F represent the same microscopic fields as A, B and C, respectively showing only the FoxJ1 and nuclear staining. Scale bar = 30 μm (G) Quantification of the percent FoxJ1 positive cells after 21 d of differentiation using ALI conditions treated with Air, Smoke or Smoke + Gefitinib. N = 7 different lung donors, * P<0.05, one-way ANOVA.