Fig 6. Inhibition of EGFR signaling promotes ciliated cell restoration after smoke exposure.

(A–D) Representative confocal micrographs of WCS treated differentiated human airway epithelial cells treated without (A, B) or with (C, D) Gefitinib for 7 d (A, C), or 14 d (B, D) after WCS treatment was stopped and stained for FoxJ1 (green), acetylated-tubulin (white) or nuclei (blue). Scale bar = 30 μm. (E) Quantification of the percent FoxJ1 positive cells cultured 7 and 14 d without (white bars) or with Gefitinib (grey bars) after WCS treatment was stopped. N = 2 for 7 d, N = 4 for 14 d. * P<0.05, Two tailed Student’s t test.