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. 2016 Aug 17;11(8):e0161438. doi: 10.1371/journal.pone.0161438

Fig 1. Detection of the BRCA2 c.156_157insAlu mutation and the BRCA1 c.3331_3334del mutation in FFPE tissue.

Fig 1

(A) Gel electrophoresis pattern of amplification of BRCA2 exon 3 (left panel) and nested PCR specific for the BRCA2 c.156_157insAlu mutation (right panel). In non-carriers of the mutation (N) only one amplicon is expected, whereas in carriers (P) a second amplicon is visible in the nested PCR. Non template control (NTC) and 100 bp DNA standard (M) also shown. (B) Capillary electrophoresis pattern from a negative sample (left panel) and a positive control of the BRCA1 c.3331_3334del mutation (right panel) showing one peak (wild-type alleles) and two peaks (wild-type and mutant allele with 4 bp deletion), respectively.