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. Author manuscript; available in PMC: 2017 Aug 28.
Published in final edited form as: Cancer Lett. 2016 May 26;379(1):60–69. doi: 10.1016/j.canlet.2016.05.029

Figure 2. HEXIM1 is required for HMBA- and 4a1- induced p21/p27 expression and differentiation.

Figure 2

MCF7 cells transfected with control siRNA or HEXIM1 siRNA were treated with vehicle (DMSO), HMBA (5 mM), or 4a1 (50 uM) for 8, 18 or 72 h. (A) Cells were processed for Western blot analyses of HEXIM1 or p21 expression or (B) stained with Nile Red to determine lipid vacoule formation (differentiation marker). MDA-MB-231 cells transfected with control or FLAG-tagged HEXIM1 expression vector or MDA-MB-468 cells transfected with control siRNA or HEXIM1 siRNA were treated with vehicle (DMSO), HMBA (5 mM), or 4a1 (50 uM) for 8, 18 or 72 h. (D) Cells were processed for Western blot analyses of HEXIM1 or p27 expression or (B) stained with Nile Red. All photographs of stained cells were taken at 40x magnification. The figures are representative of 3 experiments.