Fig 1.

(A) Breeding process of the ‘cgy-2-NIL’. RPGR = recurrent parent genome recovery. Discontinuous lines link the last generation where the definitive near isogenic lines (NILs) were selected. Numbers alongside discontinuous lines show how many lines were obtained in each candidate line. (B) Graphical genotype for chromosome 20 in ‘cgy-2-NIL’ with the ‘DN47’ genetic background. (C) SDS-PAGE analysis of mature seed proteins of DN47 and four cgy-2-NIL lines: B-12088-3, B-12088-6, B-12088-8, and B-12088-12. (D) Immunoblot analysis of the seed extracts shown in (C) using antibodies specific for β-conglycinin subunits. The sizes of the protein markers (M) in kilodaltons are shown on the left of the image in (C). The arrows point to the α′, α, and β-subunits of β-conglycinin in Fig (C) and (D).