Fig 2.

(A) Fully expanded flowers were marked individually with a tag at the 4^th, 5^th, 6^th, or 7^th nodes on cgy-2-NIL and DN47. (B) Pod samples were collected during seed development at 15, 18, 20, 25, 30, 35, 40, 45, 50, 55, and 60 days after flowering (DAF). (C) Developmental changes in morphology, size, and weight of sampled seeds in cgy-2-NIL and DN47. (D) Comparison of transcript levels of the α-subunit gene between cgy-2-NIL and DN47. Differential expression of more than 50-fold was identified during the 25–55 days after flowering (DAF) development stages. Five stages of soybean seeds collected at 18, 25, 35, 50, and 55 DAF were finally selected to subjected to RNA-seq.