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Volumes of manual (column 1) and automatic (column 2) segmentations of the substantia nigra as a function of the image intensity within the structure. Image intensities for the FLASH volumes were normalised through division by the average WM intensity. Only echo 3 is shown as results for echo 1 and 2 are very similar. Green: young, magenta: middle-aged, cyan: elderly. Regression lines are solid if significant (p ≤ 0.05) and dotted otherwise.
