Extended Data Figure 6. Temporal trend in accumulation of nonsynonymous mutations relative to the neutral expectation in nonmutator lineages.

Interval-specific accumulation of nonsynonymous mutations calculated from changes in the total number of nonsynonymous mutations between successive samples. As with the cumulative data in Fig. 5b, values are scaled by the average rate of accumulation for synonymous mutations over 50,000 generations, after adjusting for the numbers of genomic sites at risk for nonsynonymous and synonymous mutations. Each point shows the average rate calculated for a nonmutator or premutator population; small horizontal offsets were added so that overlapping points are visible. Note the discontinuous scale; populations with no additional mutations over an interval are plotted below. Colours are the same as in Fig. 1. Black lines connect grand means; the grey shading shows standard errors calculated from the replicate populations.