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. Author manuscript; available in PMC: 2017 Aug 16.
Published in final edited form as: Immunity. 2016 Aug 2;45(2):267–279. doi: 10.1016/j.immuni.2016.07.010

Figure 2. Vps33B has a conserved function in the regulation of innate immune signaling.

Figure 2

A) Quantification of transcripts encoding the indicated anti-bacterial peptides relative to the ribosomal protein RP49 before or 24 h after injection of E. coli into OreR or Vps33B5.20 mutant adult flies.

B–D) ELISA-quantification of TNFα and IL-6 cytokines after a 12-h stimulation of macrophages expressing control or Vps33B shRNA with no ligand or LPS (B), Pam3CSK4 (C) or CpG (D).

E,F) qRT-PCR quantification of IFNβ expression relative to Histone2A after stimulation of macrophages expressing control or Vps33B shRNA with LPS (E) or Poly:IC (F) for the indicated times. (ND: not detected).

Data in A, E and F are representative of 2 independent experiments and data in B–D are representative of 5 independent experiments.