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. Author manuscript; available in PMC: 2017 Aug 16.
Published in final edited form as: Immunity. 2016 Aug 2;45(2):267–279. doi: 10.1016/j.immuni.2016.07.010

Figure 3. Vps33B silencing sensitizes macrophages to low LPS doses.

Figure 3

A) ELISA measurements of IL-6 (A) and TNF-α (A’) in the supernatants of Vps33B-deficient or control macrophages exposed to the indicated concentrations of LPS for 12 hours.

B) Vps33B-silenced or control macrophages were exposed to control beads or LPS-bound beads for 6 hours. Secreted IL-6 was measured by ELISA (B) or IFNβ transcript relative to Histone2A by qRT-PCR (B’).

(“ND” indicates not detected).

C,D) Flow cytometry analysis of phospho-p38 MAP kinase at indicated time points after stimulation with 10 ng/ml (C) or 10 ng/ml of LPS (D).

Data in this figure here are representative of 3 independent experiments. Please also see Figure S2.