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. Author manuscript; available in PMC: 2017 Aug 16.
Published in final edited form as: Immunity. 2016 Aug 2;45(2):267–279. doi: 10.1016/j.immuni.2016.07.010

Figure 5. Vps33B is required for maturation of TLR4-positive endosomes in LPS-stimulated macrophages.

Figure 5

A,B) Micrographs of macrophages expressing control (A) or Vps33B shRNA (B) visualize TLR4-positive endosomes and the indicated Rabs or dextran preloaded into lysosomes, following LPS stimulation chases for the indicated times. Scale bar: 10 µm.

C–F) Quantification of the fraction of TLR4 colocalizing with the indicated Rabs or dextran-labeled lysosomes at different times after LPS stimulation. Note that the lower colocalization of TLR4 with lysosomes at 120 min in control macrophages primarily reflects the low amounts of TLR4 remaining in these cells. All data here are representative of 3 independent experiments.