Skip to main content
. Author manuscript; available in PMC: 2017 May 18.
Published in final edited form as: Acta Haematol. 2016 May 18;136(2):85–97. doi: 10.1159/000445096

Figure 5. Fibroblasts suppress CD4+ T cell proliferation.

Figure 5

PBMCs from a healthy donor were stained with CFSE. Labled PBMCs were plated with four fibroblast strains (FB) or our standard BM MSCs (positive control) at different PBMC:MSC/FB ratios. After a 4-day co-culture, cells were collected, stained with anti-CD4, and analyzed by flow cytometry. A non-stimulated (ns) co-culture at 1-0.05 PBMC:MSC/FB ratio served as a negative control as well as to measure the level of stimulation induced by the strain itself. The value of p-m/f indicates the ratio of PBMCs to MSCs/fibroblasts. Bars represent means ±SE.