Skip to main content
. Author manuscript; available in PMC: 2016 Aug 31.
Published in final edited form as: Cell Rep. 2016 Jul 28;16(7):1891–1902. doi: 10.1016/j.celrep.2016.07.006

Figure 2. Effect of deletion of eif3e on the synthesis of metabolism-related proteins.

Figure 2

A) Correlation between changes in the association of a protein with 80S complexes and its translation rate as determined by pulsed SILAC in the absence of eif3e. Translation rates are averages of two replicates. Pearson coefficient and p value of an ANOVA regression analysis are indicated.

B) 80S complexes were isolated from cells pulse-labeled with 15N-NH4Cl to profile changes in the 80S association of newly synthesized metabolism-related proteins in eif3e mutant cells relative to wild-type (WT) cells. The resulting eif3e/WT log2 ratios were plotted on the vertical axis. Data are averages of two independent experiments. Corresponding steady-state 80S association ratios were plotted on the horizontal axis. Pearson coefficient and p value of an ANOVA regression analysis are indicated.

C) Intensity map of changes (log2) in 80S association, translation (pulsed SILAC), and steady-state protein and mRNA levels eif3e deleted cells /WT ratios. Proteins are sorted according to gene ontology terms. Missing values are in light grey.