Figure 2.

Interaction between SmpB and SN by B2H. The co-transformants were spotted onto non-selective and 5 mM 3-AT selective medium by series dilution method, on which 10 μl of each dilution was spotted. (A) Evaluation of SN interacting with SmpB. (B) Evaluation of SN truncations interacting with SmpB. (C) Identification of the binding regions of SmpB interacting with SN. (D) Alignments of N- and C- terminuses of 15 SmpB sequences (Supplementary Table 3) were displayed by WebLogo (http://weblogo.berkeley.edu/logo.cgi). The mutation sites are marked by red pentagrams. (E) Identification of the key sites of SmpB interacting with SN. The conserved amino acids of SmpB were mutated and applied for testing the interaction with SN.