Table 1.
Plasmids used in this study.
| Plasmids name | Properties | Source or References |
|---|---|---|
| pBT-LGF2 | Positive control, 3.3 kb plasmid, p15A ori, lac UV-5 promoter, CamR. | Stratagene |
| pTRG-Gal11p | Positive control, 4.6 kb plasmid, ColE1 ori, lpp/lac-UV5 promoter, TetR. | Stratagene |
| pBT | Bait plasmid, 3.2 kb, p15A ori, lac UV-5 promoter, CamR. | Stratagene |
| pTRG | Prey plasmid, 4.4 kb, ColE1 ori, lpp/lac-UV5 promoter, TetR. | Stratagene |
| pBT-ArfA | pBT derivative, expresses ArfA with λcI, CamR. | This study |
| pBT (ArfA-E28IK) | pBT-ArfA derivative, mutates E28IK to AAA. | This study |
| pBT (ArfA-D31N) | pBT-ArfA derivative, mutates D31N to AA. | This study |
| pBT (ArfA-A36L) | pBT-ArfA derivative, mutates A36L to AA. | This study |
| pBT (ArfA-L42F) | pBT-ArfA derivative, mutates L42F to AA. | This study |
| pBT (ArfA-K52GK) | pBT-ArfA derivative, mutates K52GK to AAA. | This study |
| pBT (ArfA-G55SY) | pBT-ArfA derivative, mutates G55SY to AAA. | This study |
| pBT (ArfA-R59K) | pBT-ArfA derivative, mutates R59K to AA. | This study |
| pBT (ArfA-K62) | pBT-ArfA derivative, mutates K62 to P. | This study |
| pBT-SmpB | pBT derivative, expresses smpB with λ cI. | Liu et al., 2015 |
| pBT-SmpBΔN34 | pBT-SmpB derivative, deletes 34-residue at N-terminal SmpB. | Liu et al., 2015 |
| pBT-SmpB ΔN34C30 | pBT-SmpB derivative, deletes 34-residue at N-terminal and 30-residue at C-terminal SmpB. | Liu et al., 2015 |
| pBT-SmpBΔC30 | pBT-SmpB derivative, deletes 30-residue at C-terminal SmpB. | Liu et al., 2015 |
| pBT (SmpB-G11S) | pBT-SmpB derivative, mutates G11S to AA. | Liu et al., 2015 |
| pBT (SmpB-T14I) | pBT-SmpB derivative, mutates T14I to AA. | Liu et al., 2015 |
| pBT (SmpB-F26I) | pBT-SmpB derivative, mutates F26I to AA. | Liu et al., 2015 |
| pBT (SmpB-E32AG) | pBT-SmpB derivative, mutates E32AG to AAA. | Liu et al., 2015 |
| pBT (SmpB-G133K) | pBT-SmpB derivative, mutates G133K to AA. | Liu et al., 2015 |
| pBT (SmpB-D138KR) | pBT-SmpB derivative, mutates D138KR to AAA. | Liu et al., 2015 |
| pBT (SmpB-K152) | pBT-SmpB derivative, mutates K152 to P. | Liu et al., 2015 |
| pTCN-22 | 7.1-kb plasmid, expresses SN with six histidine residues, ColE1 ori, AmpR. | Norman et al., 1999 |
| pTRG-SN | pTRG derivative, expresses SN with RNAP, TetR. | This study |
| pTRG-SNΔN57 | pTRG derivative, deletes 57-residues at N-terminus of SN. | This study |
| pTRG-SN ΔN57ΔC45 | pTRG derivative, deletes 57-residues at N-terminus, and 45-residues at the C-terminus of SN. | This study |
| pTRG-SNΔC45 | pTRG derivative, deletes 45-residues at C-terminus of SN. | This study |
| pTRG-SN-peptides | pTRG derivative, expresses random peptide with RNAP, TetR. | This study |
| pTRG-PA-2 | pTRG derivative, expresses SN-PA-2 with RNAP, TetR. | This study |
| pRE112 | 5.8-kb suicide plasmid, with oriT RP4, CamR. | Edwards et al., 1998 |
| pk18mobsacB | 5.7-kb suicide plasmid, with pBR322 ori and neokanamycin promoter, NeoR, KanR. | Schäfer et al., 1994 |
| pN-NeoR | pRE112 derivative, inserted neokanamycin promoter, and NeoR of pk18mobsacB | This study |
| pN-SN | pRE112 derivative, expresses SN under control of pk18mobsacB neokanamycin promoter. | This study |
| pN-PA-2 | pRE112 derivative, expresses SN-PA-2 under control of pk18mobsacB neokanamycin promoter. | This study |