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. 2016 Aug 18;7:1283. doi: 10.3389/fmicb.2016.01283

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Copyright © 2016 Sánchez-Riego, Mata-Cabana, Galmozzi and Florencio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Oligomerization of AnNTRC and 2-Cys Prx under heat shock. (A) Non-reducing SDS-PAGE, reducing SDS-PAGE and native-PAGE Western blots of AnNTRC at 0 h and 8 h of treatment at 45°C. The AnNTRC monomer is pointed by arrows, the asterisk (^∗) marks the localization of the oligomers and “a” and “b” indicates two unspecific bands. (B) Western blot of the different fractions obtained by SEC of protein extracts from WT and ΔntrC strains before and after 8 h of treatment at 45°C. The top panel corresponds to the Western blot against AnNTRC and the bottom one to the one against 2-Cys Prx. The fractions corresponding to the different protein molecular markers used to calibrate the column are indicated in the top blot.