Table 2.
Citrullinated peptides and their deaminized sites identified by MALDI-TOF-MS in the RA SFs
| Gene name | Protein ID | Peptide sequence | Citrullinated sites |
|---|---|---|---|
| A2M | A2MG_HUMAN | DNGCFRSSGSLLNNAIK | R1081 |
| GNRIAQWQSFQLEGGLK | R174 | ||
| EQAPHCICANGRQTVSWAVTPK | R853 | ||
| FQVDNNNRLLLQQVSLPELPGEYSMK | R1297 | ||
| ACTG1 | ACTG_HUMAN | DLYANTVLSGGTTMYPGIADRMQK | R312 |
| AGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQK | R28 | ||
| ALB | ALBU_HUMAN | AWAVARLSQRFPK | R246 |
| LCTVATLRETYGEMADCCAK | R105 | ||
| VHTECCHGDLLECADDRADLAK | R281 | ||
| RMPCAEDYLSVVLNQLCVLHEK | R469 | ||
| YLYEIARRHPYFYAPELLFFAK | R169 | ||
| CCTESLVNRRPCFSALEVDETYVPK | R509 | ||
| ANXA1 | ANXA1_HUMAN | DITSDTSGDFRNALLSLAK | R177 |
| GTDVNVFNTILTTRSYPQLRRVFQK | R228 | ||
| APOA1 | APOA1_HUMAN | ENGGARLAEYHAK | R212 |
| VEPLRAELQEGARQK | R155 | ||
| DSGRDYVSQFEGSALGK | R51 | ||
| PALEDLRQGLLPVLESFK | R239 | ||
| APOB | APOB_HUMAN | LEGTTRLTRK | R3386 |
| LTTNGRFREHNAK | R1689 | ||
| AEFTGRHDAHLNGK | R3020 | ||
| GNVATEISTERDLGQCDRFK | R207 | ||
| IREVTQRLNGEIQALELPQK | R2449 | ||
| RLIDLSIQNYHTFLIYITELLK | R4519 | ||
| YTYNYEAESSSGVPGTADSRSATRINCK | R75 | ||
| ARHGAP4 | F5GZW3_HUMAN | EEQEVSWTQYTQRK | R486 |
| AERFSSRGGRLGSSREHQSFRK | R73, R77 | ||
| ELLGKTSVRQGLGPASTTSPSPGPRSPK | R889 | ||
| LREAERQEEKRAGRSVPTTTAGATEAGPLRK | R198 | ||
| ARPC1B | ARC1B_HUMAN | QSSQRGLTARERFQNLDK | R294, R299 |
| PTLVILRINRAARCVRWAPNENK | R100 | ||
| C1R | C1R_HUMAN | GFLAYYQAVDLDECASRSK | R149 |
| MQTRAGSRESEQGVYTCTAQGIWK | R420 | ||
| DCGQPRNLPNGDFRYTTTMGVNTYK | R388 | ||
| C1S | C1S_HUMAN | AARLPVAPLRK | R586 |
| C2 | CO2_HUMAN | SSGQWQTPGATRSLSK | R77 |
| C3 | CO3_HUMAN | RRHQQTVTIPPK | R880 |
| VLLDGVQNPRAEDLVGK | R315 | ||
| TVAVRTLDPERLGREGVQK | R945 | ||
| GYTQQLAFRQPSSAFAAFVK | R1060 | ||
| ITHRIHWESASLLRSEETK | R1310 | ||
| PDGVFQEDAPVIHQEMIGGLRNNNEK | R1134 | ||
| C4B | CO4B_HUMAN | ISARFSDGLESNSSTQFEVK | R218 |
| VDFTLSSERDFALLSLQVPLK | R80 | ||
| AAANQMRNFLVRASCRLRLEPGK | R1675 | ||
| SHALQLNNRQIRGLEEELQFSLGSK | R1349 | ||
| C4BPA | C4BPA_HUMAN | PELVNGRLSVDK | R493 |
| NLRWTPYQGCEALCCPEPK | R353 | ||
| C6 | CO6_HUMAN | FRCDSGRCIARK | R150 |
| RSENINHNSAFK | R289 | ||
| SSRTSNPYRVPANLENVGFEVQTAEDDLK | R225 | ||
| C9 | CO9_HUMAN | NFRTEHYEEQIEAFK | R213 |
| CAT | CATA_HUMAN | NAIHTFVQSGSHLAAREK | R522 |
| CD44 | CD44_HUMAN | NGRYSISRTEAADLCK | R41 |
| EQWFGNRWHEGYRQTPK | R407, R413 | ||
| CFH | CFAH_HUMAN | RITCRNGQWSEPPK | R1149, R1153 |
| HGGLYHENMRRPYFPVAVGK | R340 | ||
| RGYRLSSRSHTLRTTCWDGK | R1210, R1215 | ||
| AQTTVTCMENGWSPTPRCIRVK | R441 | ||
| IPCSQPPQIEHGTINSSRSSQESYAHGTK | R885 | ||
| CNMGYEYSERGDAVCTESGWRPLPSCEEK | R246 | ||
| CFHR2 | FHR2_HUMAN | SHSFRAMCQNGK | R254 |
| CFI | CFAI_HUMAN | DNERVFSLQWGEVK | R480 |
| THRYQIWTTVVDWIHPDLK | R389 | ||
| CHMP2A | CHM2A_HUMAN | MDLLFGRRK | R8 |
| DLVRTRRYVRK | R71 | ||
| CLC | LEG10_HUMAN | YQVMVNGQSSYTFDHRIK | R115 |
| CP | CERU_HUMAN | ALYLQYTDETFRTTIEK | R81 |
| NLASRPYTFHSHGITYYK | R115 | ||
| ENLTAPGSDSAVFFEQGTTRIGGSYK | R415 | ||
| DNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVK | R258 | ||
| NMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWK | R830 | ||
| CPB2 | CBPB2_HUMAN | DHEELSLVASEAVRAIEK | R342 |
| CTLA4 | CTLA4_HUMAN | AMHVAQPAVVLASSRGIASFVCEYASPGK | R51 |
| AQLNLATRTWPCTLLFFLLFIPVFCK | R18 | ||
| ENO1 | ENOA_HUMAN | TGAPCRSERLAK | R403 |
| LAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIK | R372 | ||
| F2 | THRB_HUMAN | YTACETARTPRDK | R94, R97 |
| DSTRIRITDNMFCAGYK | R541 | ||
| FF3 | AFF3_HUMAN | DFLTDRSNQSHLVGVPK | R111 |
| EAAANGGSGPRAPVGSINARTTSDIAK | R745 | ||
| YTSEDLTSSSRPNGNSLFTSASSSK | R926 | ||
| SPPAAVAVAVSAAAPPPAVPCAPAENAPAPARRSAGK | R606 | ||
| FGA | FIBA_HUMAN | NVRAQLVDMK | R160 |
| GLIDEVNQDFTNRINK | R84 | ||
| FGB | FIBB_HUMAN | REEAPSLRPAPPPISGGGYRARPAK | R60, R72 |
| EDGGGWWYNRCHAANPNGRYYWGGQYTWDMAK | R445 | ||
| FGG | FIBG_HUMAN | YEASILTHDSSIRYLQEIYNSNNQK | R134 |
| YTGNTYRVGDTYERPK | R106 | ||
| WLPSSSPVTGYRVTTTPK | R1573 | ||
| DNRGNLLQCICTGNGRGEWK | R265 | ||
| GC | VTDB_HUMAN | HLSLLTTLSNRVCSQYAAYGEK | R218 |
| HQPQEFPTYVEPTNDEICEAFRK | R149 | ||
| RSDFASNCCSINSPPLYCDSEIDAELK | R445 | ||
| H1FX | H1X_HUMAN | VPWFDQQNGRTYLK | R86 |
| YSQLVVETIRRLGERNGSSLAK | R57, R62 | ||
| H2AFY | H2AY_HUMAN | SIAFPSIGSGRNGFPK | R318 |
| HABP2 | HABP2_HUMAN | EEFHEQSFRVEK | R391 |
| FCEIGSDDCYVGDGYSYRGK | R203 | ||
| LIANTLCNSRQLYDHMIDDSMICAGNLQK | R480 | ||
| HIST2H2AC | H2A2C_HUMAN | TRIIPRHLQLAIRNDEELNK | R89 |
| GNYAERVGAGAPVYMAAVLEYLTAEILELAGNAARDNK | R43, R72 | ||
| HMGB2 | HMGB2_HUMAN | MSSYAFFVQTCREEHK | R24 |
| HNRNPA1L2 | RA1L2_HUMAN | GGNFGGRSSGPYGGGGQYFAK | R284 |
| HP | HPT_HUMAN | VSVNERVMPICLPSK | R261 |
| YVMLPVADQDQCIRHYEGSTVPEK | R311 | ||
| HPR | HPTR_HUMAN | VLVNERVMPICLPSK | R203 |
| HPX | HEMO_HUMAN | NFPSPVDAAFRQGHNSVFLIK | R102 |
| HSP90AA1 | Q8TBA7_HUMAN | AQALRDNSTMGYMAAK | R620 |
| HNDDEQYAWESSAGGSFTVRTDTGEPMGRGTK | R173 | ||
| HSPA1A | HSP71_HUMAN | LLQDFFNGRDLNK | R357 |
| EIAEAYLGYPVTNAVITVPAYFNDSQRQATK | R155 | ||
| HSPA5 | GRP78_HUMAN | SDIDEIVLVGGSTRIPK | R368 |
| RLIGRTWNDPSVQQDIK | R98 | ||
| Ig kappa chain V-II region RPMI 6410 | KV206_HUMAN | VSNRDSGVPDRFSGSGSGTDFTLK | R79 |
| Ig lambda chain V-II region NEI | LV202_HUMAN | RPSGVSNRFSGSK | R56, R63 |
| Ig lambda chain V-II region NIG-84 | LV211_HUMAN | LLIYDVNSRPSGISNRFSGSK | R56, R63 |
| IGHA1 | IGHA1_HUMAN | YLTWASRQEPSQGTTTFAVTSILRVAAEDWK | R282 |
| IGHG3 | IGHG3_HUMAN | SCDTPPPCPRCPEPK | R128 |
| TPLGDTTHTCPRCPEPK | R113 | ||
| ING4 | ING4_HUMAN | WFCPRCSQERK | R241 |
| ITIH2 | ITIH2_HUMAN | RLSNENHGIAQRIYGNQDTSSQLK | R475 |
| TILDDLRAEDHFSVIDFNQNIRTWRNDLISATK | R356, R359 | ||
| KNG1 | KNG1_HUMAN | ICVGCPRDIPTNSPELEETLTHTITK | R268 |
| KRT33B | KT33B_HUMAN | ETMQFLNDRLASYLEK | R66 |
| LBR | LBR_HUMAN | EARREVEVK | R111 |
| PLTSFRQRK | R61 | ||
| SARRSASASHQADIK | R96 | ||
| ELAVRTFEVTPIRAK | R195 | ||
| APRNDLSPASSGNAVYDFFIGRELNPRIGTFDLK | R353 | ||
| LCP1 | PLSL_HUMAN | GDEEGVPAVVIDMSGLREK | R316 |
| ALENDPDCRHVIPMNPNTNDLFNAVGDGIVLCK | R141 | ||
| LGALS3BP | LG3BP_HUMAN | SGGSDRTIAYENK | R514 |
| SQLVYQSRRGPLVK | R436 | ||
| LRG1 | A2GL_HUMAN | ALGHLDLSGNRLRK | R175 |
| LQVLGKDLLLPQPDLRYLFLNGNK | R239 | ||
| MAPRE1 | MARE1_HUMAN | PLTSSSAAPQRPISTQRTAAAPK | R168 |
| MMP8 | MMP8_HUMAN | FYQLPSNQYQSTRK | R52 |
| MNDA | MNDA_HUMAN | INQEEVGLAAPAPTARNK | R119 |
| NCF1 | NCF1_HUMAN | SGQDVSQAQRQIK | R292 |
| STATDITGPIILQTYRAIANYEK | R162 | ||
| ORM1 | A1AG1_HUMAN | EQLGEFYEALDCLRIPK | R167 |
| PABPC1 | PABP1_HUMAN | AVTEMNGRIVATK | R356 |
| PVRIMWSQRDPSLRK | R89, R94 | ||
| ITGMLLEIDNSELLHMLESPESLRSK | R604 | ||
| PADI2 | PADI2_HUMAN | VGVFYVENPFFGQRYIHILGRRK | R225, R233 |
| PADI4 | PADI4_HUMAN | GFRLLLASPRSCYK | R495 |
| TLREHNSFVERCIDWNRELLK | R536 | ||
| PFGPVINGRCCLEEK | R609 | ||
| POFUT2 | OFUT2_HUMAN | VFVATDAVRK | R337 |
| DFIWGHRQDVPSLEGAVRK | R315 | ||
| PPIA | PPIA_HUMAN | EGMNIVEAMERFGSRNGK | R148 |
| PRG4 | PRG4_HUMAN | DQYYNIDVPSRTARAITTRSGQTLSK | R1386, R1391 |
| PRKCD | KPCD_HUMAN | QSMRSEDEAK | R132 |
| SPRDYSNFDQEFLNEK | R628 | ||
| IIGRCTGTAANSRDTIFQK | R216 | ||
| PTPN22 | PTN22_HUMAN | GPRNPPPTWNI | R499 |
| PAESVQSNNSSSFLNFGFANRFSK | R491 | ||
| SAA2 | SAA2_HUMAN | RGPGGAWAAEVISNARENIQRLTGRGAEDSLADQAANK | R80, R89 |
| SERPINA3 | AACT_HUMAN | ADLSGITGARNLAVSQVVHK | R350 |
| SERPINC1 | ANT3_HUMAN | LVSANRLFGDK | R177 |
| IPEATNRRVWELSK | R78 | ||
| ANSRFATTFYQHLADSK | R89 | ||
| SLC22A4 | S22A4_HUMAN | VPLTTSLFFVGVLLGSFVSGQLSDRFGRK | R166 |
| STAT4 | STAT4_HUMAN | NVSTLSNRRFVLCGTNVK | R173 |
| SLQSSSVSERQRNVEHKVAAIK | R139 | ||
| FHGNPMHVAVVISNCLREERRILAAANMPVQGPLEK | R110 | ||
| TAGLN2 | TAGL2_HUMAN | MANRGPAYGLSREVQQK | R4 |
| TF | TRFE_HUMAN | CSTSSLLEACTFRRP | R696 |
| EGYYGYTGAFRCLVEK | R541 | ||
| ADRDQYELLCLDNTRK | R239, R251 | ||
| TNC | TENA_HUMAN | NGRENFYQNWK | R2033 |
| RVTTTRLDAPSQIEVK | R802 | ||
| VEAARNLTVPGSLRAVDIPGLK | R1127 | ||
| PDTEYEVSLISRRGDMSSNPAK | R878 | ||
| ETFTTGLDAPRNLRRVSQTDNSITLEWRNGK | R897 | ||
| TNC | TENA_HUMAN | VPEITRTVSGNTVEYALTDLEPATEYTLRIFAEK | R1866 |
| TNFAIP6 | TSG6_HUMAN | NFLAGRFSHL | R273 |
| VIM | VIME_HUMAN | MALDIEIATYRK | R401 |
| PDLTAALRDVRQQYESVAAK | R270, R273 | ||
| VPRPB | VPRBP_HUMAN | FISGTPRRK | R707 |
| SPFGSSFRTFNATDYK | R1334 |
Citrullinated residues are indicated with a bold R